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目的分析某地区献血人群乙型肝炎病毒(HBV)携带者基因型和耐药性基因变异的情况。方法选取本市2014年4月1日~12月31日22 539份无偿献血者血液标本,使用ELISA双试剂检测筛查出201份双阳性标本,然后通过荧光定量PCR筛查出99例HBV阳性标本,对其中45例病毒载量大于1 000 IU/ml的标本进行HBV耐药基因检测(PCR测序法)。分析本市HBV携带者基因型与耐药片段变异情况及其关联。结果 45例全部为乙肝野生型,无耐药突变。乙肝分型结果显示45例中有43例为B型,占95.6%,2例为C型,占4.4%。乙肝B型的分型率明显高于C型的分型率,具有统计学意义(χ2=9.256,P<0.05)。结论本市献血人群中HBV携带者的基因分型以B型为主,耐药性基因主要是野生型,荧光定量PCR阳性的标本中有45.5%的病毒载量高于1 000 IU/ml,需要跟踪治疗,为预防和治疗乙型肝炎病毒感染提供可靠依据。
Objective To analyze the genotype and drug resistance gene variation of hepatitis B virus (HBV) carriers in blood donors in a certain area. Methods 22 539 blood samples were collected from 22 539 unpaid donors from April 1 to December 31, 2014. 201 double positive samples were screened by ELISA and 99 positive samples were detected by fluorescence quantitative PCR Samples of which 45 cases of viral load greater than 1 000 IU / ml specimens for HBV resistance gene detection (PCR sequencing). To analyze the variation of HBV genotypes and drug-resistant fragments in HBV carriers in this city and their correlations. Results 45 cases were all hepatitis B wild type, no drug-resistant mutation. Hepatitis B typing showed that 43 of 45 cases were type B, accounting for 95.6%, and 2 were type C, accounting for 4.4%. The typing rate of type B hepatitis B was significantly higher than that of type C, with statistical significance (χ2 = 9.256, P <0.05). Conclusion The genotypes of HBV carriers in blood donors in this Municipality are type B and the gene of resistance is mainly wild type. 45.5% of the samples with positive fluorescence quantitative PCR are higher than 1 000 IU / ml, The need for follow-up treatment for the prevention and treatment of hepatitis B virus infection provides a reliable basis.