In previous papers, we reported the isolation of four triterpenoid saponins: tubeimo-sides I,II,III, and IV from the bulbs of Bolbostemma paniculatum Maxim franquet(Cucurbi-taceae) and characterization of tubeimoside I. In this communication we continue to reportthe structural elucidation of tubeimosides II(1), III (4), and IV (8). All of them are newsaponins,1 and 4 are novel macrocyclic saponins. Tubeimoside II(1), C_63H_98O_30, [α]_D -6.0°(c,0.32, pyridine), was obtained as colorlessamorphous powder. Its SIMS showed the [M+H]~+ion at m/z 1335. The acidic hydrolysis of1 gave polygalacic acid(2) and four sugars: glucose, rhamonse,arabinose and xylose in a mole In previous papers, we reported the isolation of four triterpenoid saponins: tubeimo-sides I, II, III, and IV from the bulbs of Bolbostemma paniculatum Maxim franquet (Cucurbi-taceae) and characterization of tubeimoside I. In this communication we continue to reportthe Structural elucidation of tubeimosides II (1), III (4), and IV (8). All of them are newsaponins, 1 and 4 are novel macrocyclic saponins. Tubeimoside II (1), C_63H_98O_30, [a] _D -6.0 ° ( Its SIMS showed the [M + H] ~ + ion at m / z 1335. The acidic hydrolysis of 1 gave polygalacic acid (2) and four sugars: glucose, rhamonse, arabinose and xylose in a mole