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本文报告了有关免疫接种、提纯和保护性磷酸缓冲液生化分析方面不同指标的资料。收集的曼氏血吸虫成虫迅速用pH6.8、0.15M PBS漂洗2次,取1g虫冰冻贮存于10ml PBS中至少10天制备成虫浸出液,经冻融、过滤、4℃下10000g离心60分钟,将悬液(盐水浸出液,SE)再通过0.22μ微孔膜过滤,其蛋白浓度用Lowry’s法测定。取SE样本用交联葡聚糖G-100柱层析获得纯化的成虫部分(FI)。将SE-CFA(福氏完全佐剂)、FI-CFA CE-C.parvum(短小棒状杆菌)或单纯SE、FI抗原分别接种雄性新
This article reports on different indicators of biochemical analysis of immunization, purification and protective phosphate buffered saline. Adult Schistosoma mansoni collected rapidly rinsed twice with pH 6.8 and 0.15 M PBS, and 1 g of insect was stored frozen in 10 ml of PBS for at least 10 days to prepare adult leachate. After freeze-thaw, filtration and centrifugation at 10,000 g for 60 minutes at 4 ° C, The suspension (saline leachate, SE) was then filtered through a 0.22 μ microporous membrane and the protein concentration determined by Lowry’s method. The SE samples were taken and the purified adult part (FI) was obtained by Sephadex G-100 column chromatography. SE-CFA (Freund’s complete adjuvant), FI-CFA CE-C.parvum (Corynebacterium parvum), or pure SE, FI antigen were inoculated into male new