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目的 :研究多种生长因子及其受体在急性放射性皮肤溃疡组织中的表达及对溃疡形成和发展的影响。方法 :采用雌性Wistar大鼠 ,以手术法建立单纯皮肤伤口动物模型 ,以60 Coγ射线局部照射法建立急性放射性皮肤溃疡动物模型。观察病变 5 5d ,采用免疫组化、原位杂交和图像分析等方法检测单纯伤口及皮肤溃疡组织中表皮生长因子 (EGF) ,碱性成纤维细胞生长因子 (bFGF) ,血管内皮生长因子 (VEGF) ,血小板衍生生长因子_B(PDGF_B) ,转化生长因子_β1(TGF_β1)等及其受体的转录和表达水平。结果 :照后 14d照射野内开始出现皮肤溃疡 ,之后逐渐扩大、融合、加深。皮肤受照射区多种细胞 ,特别是溃疡床表皮细胞、成纤维细胞及血管内皮细胞中EGF ,bFGF ,PDGF_B ,VEGF等因子及其受体的转录及表达水平均较正常皮肤组织有所增强 ,但与单纯伤口组比较 ,溃疡组织上述因子及其受体的转录及表达水平明显降低。TGF_β1及TGF_βR1在受照射皮肤中表达增强 ,溃疡床的阳性细胞数量较单纯伤口中减少 ,强度未见减弱。结论 :急性放射性皮肤溃疡组织中多种生长因子及其受体表达水平降低可能与放射性皮肤溃疡发生、发展及难愈合 (不能形成有效肉芽组织 )的分子机制相关
Objective: To study the expression of multiple growth factors and their receptors in acute radiation-induced skin ulcers and their effects on the formation and development of ulcers. Methods: The Wistar rats were used to establish the animal models of skin wounds by surgery. The animal model of acute radiation-induced skin ulcers was established by 60Co γ ray irradiation. The lesions were observed for 5 days and the expressions of epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) were detected by immunohistochemistry, in situ hybridization and image analysis. ), Platelet-derived growth factor-B (PDGF_B), transforming growth factor-β1 (TGF-β1) and their receptors. Results: 14d after irradiation began to appear in the field of skin ulcers, and then gradually expanded, integration, and deepen. The transcription and expression levels of EGF, bFGF, PDGF_B, VEGF and other factors and their receptors in many irradiated cells, especially in ulcerated epithelial cells, fibroblasts and vascular endothelial cells, were significantly higher than those in normal skin, However, compared with the simple wound group, the transcription and expression levels of these factors and their receptors in ulcer tissues were significantly lower. The expression of TGF-β1 and TGF-βR1 in the irradiated skin was enhanced. The number of positive cells in the ulcer bed was less than that in the pure wound and the intensity was not reduced. CONCLUSIONS: The decreased expression of multiple growth factors and their receptors in acute radiation-induced skin ulcers may be related to the molecular mechanism of the occurrence and development of radiation-induced skin ulcers and the difficulty of healing (not forming effective granulation tissue)