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目的:比较普萘洛尔(Pro)药物应用与否时,苯福林(Phe)对DAD与TA的不同影响.方法:绵羊浦肯野纤维细胞内微电极记录跨膜电位法.结果:Phe 1 μmol·L~(-1)灌流60 min,无Pro时,使DAD幅值由7.5±1.2增加到9.0±2.0 mV(n=8,P<0.05),DAD时程变化不显著,3/11可诱发TA;Pro 0.5 μmol·L~(-1)阻断μ受体时,前20 min使DAD幅值由7.2±1.8增加到8.3±2.1 mV(n=8,P<0.01),而后减少至6.35=1.6 mV,DAD时程从灌流前192±17延长至280±27 ms(n=8,P<0.01);同时对乙酰毒毛旋花子苷元诱发的触发活动有抑制,并可被哌唑嗪所阻断.结论:在Pro有效消除儿茶酚胺致使心律失常发生中,除阻断β受体直接作用外,相应加强α受体激动对DAD与TA的抑制亦是其中重要原因之一.
OBJECTIVE: To compare the different effects of phenylephrine (Phe) on DAD and TA when propranolol is used or not.Methods: Transcutaneous membrane potential method was used to record the microelectrode potential of Purkinje fiber in sheep.Results: Phe DAD amplitude was increased from 7.5 ± 1.2 to 9.0 ± 2.0 mV (n = 8, P <0.05) in the absence of Pro at 1 μmol·L -1 perfusion, 11 induced TA; Pro 0.5 μmol·L -1 block μ receptor, the amplitude of DAD increased from 7.2 ± 1.8 to 8.3 ± 2.1 mV (n = 8, P <0.01) To 6.35 = 1.6 mV. The time course of DAD prolonged from 192 ± 17 to 280 ± 27 ms before perfusion (n = 8, P <0.01). At the same time, the triggering activity of acetyl viologenin was inhibited, Was blocked by prazosin.Conclusion: In addition to the direct effect of blockade of β receptor in the effective elimination of catecholamine-induced arrhythmias, the corresponding inhibition of DAD and TA by α receptor activation is also one of the important reasons .