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目的:建立测定复方救心片中华蟾酥毒基、酯蟾毒配基含量的方法。方法:采用高效液相色谱法,色谱柱为Phenomenex Luna C18(150mm×4.6mm,5μm)柱,流动相为乙腈-0.5%磷酸二氢钾溶液(50∶50,用磷酸调节pH为3.2),流速0.6mL.min-1,检测波长296nm,柱温30℃。结果:华蟾酥毒基进样量在0.33～1.65μg(r=0.9999)范围内线性关系良好;平均回收率(n=6)为99.96%,RSD为1.1%。酯蟾毒配基进样量在0.42～2.09μg(r=0.9999)范围内线性关系良好;平均回收率(n=6)为98.35%,RSD为1.3%。结论:本方法操作简便,结果准确可靠,重复性好。
OBJECTIVE: To establish a method for determination of content of toadstool base and ester glycosides in Fufang Jiuxin Tablets. Method: HPLC was used. The column was Phenomenex Luna C18 (150mm×4.6mm, 5μm) column. The mobile phase was acetonitrile-0.5% potassium dihydrogen phosphate solution (50:50, pH was adjusted to 3.2 with phosphoric acid). The flow rate was 0.6 mL.min-1, the detection wavelength was 296 nm, and the column temperature was 30°C. RESULTS: The linear relationship was good in the range of 0.33-1.65 μg (r=0.9999). The average recovery rate (n=6) was 99.96% and the RSD was 1.1%. The linear relationship was good in the range of 0.42～2.09μg (r=0.9999). The average recovery (n=6) was 98.35% and the RSD was 1.3%. Conclusion: This method is simple, accurate, reliable and reproducible.