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利用穗部性状存在明显差异的粳稻TD70和籼稻品种Kasalath杂交,经单粒传法获得的240个重组自交系(RIL)为作图群体,分别于2010和2011年对穗长每穗总粒数和着粒密度进行鉴定,用完备区间作图法,以均匀分布于12条染色体的141个SSR标记对粒型性状进行QTL检测结果表明,共检测到3个性状的QTL23个,其中控制穗长的5个QTL每穗总粒数的8个QTL着粒密度的10个QTL,分布在第2、3、4、6、7、8、9和10染色体上,LOD值范围为2.5~9.3,单个QTL的贡献率介于4.0%~20.8%之间第2染色体的RM5699-RM424第3染色体的RM489-RM1278第4染色体的RM3367-RM1018和第6染色体的RM3343-RM412区间,都检测到同时影响每穗总粒数着粒密度的QTL,2年均被检测到的QTL共有6个QTL,分别是控制穗长的qPL3每穗总粒数的qTSP4、qTSP6-2、qTSP7着粒密度的qGD3-2、qGD7其中qPL3qTSP6-2qGD3-2和qGD7为主效QTL除穗长性状5个位点均有报道外,其余2个性状中均发现新的位点,共有16个QTL可能是新的位点,单个QTL贡献率为4.0%~9.5%本研究为进一步精细定位或克隆这些粒型QTL奠定了基础。
In this study, 240 recombinant inbred lines (RILs) derived from a cross between indica rice TD70 and indica variety TD70, which were significantly different in panicle traits, were used as mapping populations. Number and grain density. The QTLs for grain traits were detected by complete interval mapping using 141 SSR markers evenly distributed on 12 chromosomes. The results showed that 23 QTLs for 3 traits were detected, of which, Eight QTLs for the total number of five QTLs per panicle were mapped on chromosomes 2, 3, 4, 6, 7, 8, 9 and 10 with LOD values ranging from 2.5 to 9.3 , The contribution rate of single QTL ranged from 4.0% to 20.8%. RM3699-RM424 on chromosome 2 RM489-RM1278 on chromosome 4 RM3367-RM1018 on chromosome 4 and RM3343-RM412 on chromosome 6 were detected simultaneously There were 6 QTLs affecting the total grain-per-grain densitometry (QTL) per panicle, qTL3, qTSP4-2 and qTSP7-qGD3 -2, qGD7 Among them, qPL3qTSP6-2qGD3-2 and qGD7 were the major QTLs, except for the five long and short traits reported in the other two traits Loci, QTL may be a total of 16 new sites, the contribution of a single QTL was 4.0% -9.5% of the fine mapping study or cloning these QTL grain type basis.