１引言蛋白氨基酸在水溶液中以两性离子存在，不具有挥发性，高温易分解，需先将其衍生化，生成挥发性好且热稳定的衍生物，才能进行气相色谱分析。通常采用的方法是羧基酯化和氨基酰化，反应的时间和温度都将影响氨基酸的衍生化程度，因此必须确定出最佳反应条件。有关上述四 1 Introduction Protein amino acids exist as zwitterions in aqueous solution, do not have volatility, and are easily decomposed at high temperatures. They need to be derivatized first to generate volatile and thermally stable derivatives for gas chromatographic analysis. Commonly used methods are carboxyl esterification and aminoacylation, the reaction time and temperature will affect the degree of amino acid derivatization, it is necessary to determine the best reaction conditions. About the above four