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作者将编码乙型肝炎病毒表面抗原(HBs Ag) Xho /Bgl 的片段克隆至质粒p CI,用限制性酶 Xho 或 Bam H 切割 ,构建成在人巨细胞病毒 (HCMV)立即早期启动子控制下表达 HBs Ag编码基因的质粒p CI/S。另将含 Kpn /Nru 片段的结蛋白启动子 /增强子序列克隆至缺乏 CMV的p CI/S载体 ,
The authors cloned a fragment of Xho / Bgl encoding hepatitis B virus surface antigen (HBs Ag) into plasmid p CI and cleaved it with the restriction enzyme Xho or Bam H to construct a plasmid that is under the control of the immediate early promoter of human cytomegalovirus (HCMV) Plasmid p CI / S expressing HBsAg-encoding gene. The desmin promoter / enhancer sequence containing the Kpn / Nru fragment was cloned into the p CI / S vector lacking the CMV,