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目的探讨雷帕霉素药物支架体外加速释放条件。方法雷帕霉素药物支架体外加速释放条件采用蠕动泵循环装置模拟血流环境,溶出介质选择2%十二烷基硫酸钠+10%乙腈+88%PBS缓冲液。高效液相色谱法测定药物释放,比较摇床法28 d释放结果与加速释放法1 d释放结果的相关性。结果摇床法28 d后药物累计释放了70%,加速释放法1 d后药物累计释放了79%,两者都经历了初期的快速释放阶段和后期的平缓释放阶段。结论建立雷帕霉素药物支架体外加速释放条件,加速释放法与摇床法的结果具有相关性。
Objective To investigate the accelerated release of rapamycin scaffold in vitro. Methods Accelerated release conditions of rapamycin scaffolds in vitro The peristaltic pump circulation device was used to simulate the blood flow environment. The dissolution medium was selected with 2% sodium dodecyl sulfate + 10% acetonitrile + 88% PBS buffer. The drug release was determined by high performance liquid chromatography (HPLC), comparing the 28 d release of shaker method with the 1 d release of accelerated release method. Results The total drug release was 70% after 28 days of shaker method and 79% after 1 d of accelerated release method. Both of them experienced the initial rapid release stage and the late gentle release stage. Conclusion The establishment of rapamycin drug stent in vitro accelerated release conditions, accelerated release method and shaker method results are relevant.