作者构建了单独编码乙型脑炎病毒(JEV)包膜 (E)糖蛋白的 DNA质粒 p CMX-ENV,该质粒转染 Vero细胞后可在细胞内表达 E蛋白。作者就其在小鼠肌内 (im)和鼻内 (in)接种对脑内 (ic) JEV攻击的防御作用进行了研究。　　对 4～ 6周龄异系交配瑞士雄性小鼠间隔两周在四头肌 im接种 5 0 The authors constructed a DNA plasmid, pCMX-ENV, that encodes the glycoprotein of the Japanese Encephalitis virus (JEV) envelope (E) alone and expresses the E protein intracellularly after transfection into Vero cells. The authors studied their defensive effects on intracerebral (ic) JEV challenge with intramuscular (im) and in (in) inoculation in mice. Male mice of 4 to 6 weeks of age who were cross-bred were im-vaccinated in quadriceps two weeks apart