Study on culturing Trichodema mutants

来源 :浙江大学学报(农业与生命科学版) | 被引量 : 0次 | 上传用户:qiminfenglin1
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Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone + chlorothalonil, or maneb or phosethyl-Al) . The pH values of media were 5, 6, 7 and 8 and hyphae were grown at temperatures of 15, 20, 25 and 30 ℃. After being cultured for 3, 4, 5, or 6 days, the strains were transferred at a lower temperature to sporulate (20 ℃) . Obtained data were analyzed statistically, with the orthogonal array and ranges (R) differing dependes on the treatments (R=40.0,42.4, 48.0, 62.8, 107.0).. The results indicated that the most important factor was the nature of the strain (R= 107.0),. while the change in temperature and time of cultivation produced the lowest effect (R= 40.0).. Each factor variance was significant and A 3B 4C 2D 1E 3 was the optimum combined condition, in which strain T1010 grew more quickly and sporulated most. Trichodema mutants strain T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg / kg (CK or procymidone + chlorothalonil, or maneb or phosethyl-Al) , 6, 7 and 8 and hyphae were grown at temperatures of 15, 20, 25 and 30 ° C. After being cultured for 3, 4, 5, or 6 days, the strains were transferred at a lower temperature to sporulate (20 ° C.) Obtained data were analyzed statistically, with the orthogonal array and ranges (R) differing dependes on the treatments (R = 40.0, 42.4, 48.0, 62.8, 107.0) .. The results indicated that the most important factor was the nature of the strain (R = 107.0), while the change in temperature and time of cultivation produced the lowest effect (R = 40.0) .. Each factor variance was significant and A 3B 4C 2D 1E 3 was the optimum combined condition, which strain T1010 more quickly and sporulated most.
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