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目的研究肝郁对女性排卵功能及血清IL-1β、IL-6的含量的影响,初步探讨肝郁与排卵功能障碍的关系及舒肝中药的作用机理。方法复制慢性应激肝郁大鼠模型,将60只性周期表现正常的大鼠随机分为5组,即正常组、模型组、阳性组、舒肝补肾高组、舒肝补肾低组。造模的同时,正常组、模型组和阳性组灌以等量蒸馏水,舒肝补肾高、低组分别灌以舒肝补肾方浸膏稀释液高剂量(生药浓度约3.744 g/mL)和低剂量(生药浓度约1.872 g/mL),连续造模及给药20 d。造模第18 d阳性组每只大鼠腹腔注射HMG40IU,48 h后再腹腔注射HCG50IU,24 h后处死。其余各组于造模第20 d经阴道涂片观察挑选处于动情期的大鼠并间隔24 h处死,进行指标测定。动态观察大鼠动情周期的变化;解剖取出输卵管并捡出卵子,镜下观察卵子形态,初步评估卵子质量;对照观察卵巢组织形态学的改变;用放射免疫法检测大鼠血清IL-1β、IL-6的含量。结果同正常组相比,模型组大鼠的性周期出现不同程度的紊乱,卵子质量较差,卵巢组织形态异常,血清IL-1β及IL-6的含量显著降低(P<0.05)。同模型组相比,舒肝补肾高、低组大鼠的紊乱性周期得到改善,卵子质量有一定提高,卵巢组织的异常形态得到一定纠正,血清IL-1β含量显著升高(P<0.05),IL-6的含量无显著差异(P>0.05)。结论舒肝补肾方能明显改善大鼠性周期的紊乱,并在一定程度上提高卵子成熟度,改善卵巢的异常形态,有效升高大鼠血清IL-1β水平,促进卵子发育成熟和排卵,为临床运用舒肝补肾法治疗肝郁型排卵功能障碍提供了有价值的客观依据。
Objective To study the effect of liver depression on the ovulation function and serum levels of IL-1β and IL-6 in female and to explore the relationship between liver depression and ovulation dysfunction and the mechanism of Shugan Chinese medicine. Methods Chronic stress liver cirrhosis rat model was duplicated. Sixty normal rats of normal sexual cycle were randomly divided into five groups: normal group, model group, positive group, Shugan Bushen Gao group and Shugan Bushen low group. At the same time of modeling, the rats in normal group, model group and positive group were treated with high dose (crude drug concentration of about 3.744 g / mL) and low Dose (crude drug concentration of about 1.872 g / mL), continuous modeling and administration of 20 d. At the 18th day, each rat was injected intraperitoneally with HMG40IU, and injected intraperitoneally with HCG50IU 48 hours later, then sacrificed 24 hours later. The remaining groups in the modeling of the 20th day by vaginal smears selected estrus rats were killed at intervals of 24 h, the index was measured. The changes of estrous cycle in rats were observed dynamically. The oviducts were taken out and the eggs were taken out. The morphology of the eggs was observed microscopically. The quality of the eggs was evaluated preliminarily. The morphological changes of the ovaries were observed by radioimmunoassay. The levels of IL-1β, IL -6 content. Results Compared with normal group, the sexual cycle of rats in the model group showed different degrees of disorder, the quality of the egg was poor, the morphology of the ovary was abnormal, and the levels of IL-1β and IL-6 in the model group were significantly decreased (P <0.05). Compared with the model group, Shugan Bushen high and low groups of rats improved the disorder cycle, the quality of the egg increased to a certain extent, the abnormal morphology of ovarian tissue was corrected, serum IL-1β content was significantly increased (P <0.05) , IL-6 content was no significant difference (P> 0.05). Conclusions Shugan Bushen Decoction can significantly improve the disorder of sexual cycle in rats and improve the maturation of ovum to a certain extent, improve the abnormal morphology of ovary, raise the level of IL-1β in serum and promote the development and ovulation of ovum The use of Shugan kidney method for treatment of liver depression ovulation dysfunction provides a valuable objective basis.