论文部分内容阅读
目的 研究木瓜苷 (GCS)预防用药对小鼠胶原性关节炎 (collagen inducedarthritis,CIA)的影响 ,并探讨其初步机制。方法 昆明种小鼠随机分为 6组 :正常对照组、模型组、木瓜苷三个剂量组和青藤碱组 (SIN) ;鸡Ⅱ型胶原免疫小鼠诱导继发性关节炎 ;足爪容积测定法、关节炎评分法和跖曲踝关节疼痛评分法观察关节炎的发生情况 ;观察胸腺指数、脾脏指数变化 ;3 H TdR参入法检测T、B细胞增殖反应 ;IL 1、IL 2活性的检测采用小鼠淋巴细胞增殖法 ;ELISA法检测血清中抗Ⅱ型胶原抗体水平 ;分光光度法测定局部踝关节PGE水平。结果 小鼠免疫后d 2 4 ,足爪出现红肿 ,继发性炎症高峰期在d 36 ,4 0后炎症逐渐减轻。GCS(6 0、12 0、2 4 0mg·kg-1)和SIN(10 0mg·kg-1)ig对CIA小鼠继发性关节炎有明显的抑制作用 ,降低CIA小鼠踝关节中增高的PGE ;CIA小鼠脾脏指数增加 ,而胸腺指数未见明显的改变 ,GCS能降低增加的CIA小鼠脾脏指数 ,对胸腺指数无明显影响 ;GCS(6 0、12 0、2 4 0mg·kg-1)和SIN (10 0mg·kg-1)使CIA小鼠增高的ConA和LPS诱导的T细胞和B细胞增殖反应降低 ;使T细胞和PMΦ分别产生的高水平IL 2和IL 1降至正常范围 ;并降低CIA小鼠血清中升高的抗CⅡ抗体。结论 GCS和SIN具有明显的抗炎作用 ,此作用可能是通过抑制
Objective To study the effect of Glycosides (GCS) prophylaxis on collagen induced arthritis (CIA) in mice and explore its preliminary mechanism. Methods Kunming mice were randomly divided into 6 groups: normal control group, model group, three doses of papaya group and sinomenine group (SIN); chicken type II collagen-immunized mice induced secondary arthritis; paw volume Measurements of arthritis, arthritis scores, and knee joint pain scores were performed to observe the occurrence of arthritis; changes in thymus index and spleen index were observed; 3 H TdR incorporation assay was used to detect T, B cell proliferation; IL 1, IL 2 activity The mouse lymphocyte proliferation assay was used for detection; anti-collagen II antibody levels in serum were detected by ELISA; and the PGE levels in local ankle joints were measured by spectrophotometry. Results After immunization, mice developed redness on d 2 4 , and inflammation was gradually reduced after d 36 and 40 on the peak of secondary inflammation. GCS (60, 120, 240 mg·kg-1) and SIN (100 mg·kg-1) ig significantly inhibited secondary arthritis in CIA mice and reduced the increase in ankle in CIA mice. The PGE; CIA mouse spleen index increased, but no significant changes in the thymus index, GCS can reduce the spleen index of CIA mice, no significant effect on the thymus index; GCS (60,120,240 mg·kg -1) and SIN (10 mg·kg-1) decreased the proliferative responses of T cells and B cells induced by ConA and LPS in CIA mice; and decreased the high levels of IL 2 and IL 1 produced by T cells and PMΦ respectively. Normal range; and decreased anti-CII antibodies in serum of CIA mice. Conclusion GCS and SIN have obvious anti-inflammatory effects, which may be inhibited by