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80年代化学和免疫学领域中的一项重大发现——催化抗体(抗体酶),把抗体的极其多样性与酶的巨大催化能力等特性结合在一起,为传统的化学和免疫学研究开辟了一个崭新的方向.利用经过特殊设计并合成的有机分子作为半抗原,联接载体蛋白后免疫动物并经细胞杂交技术,从筛选和分离的单克隆抗体中,成功地获得具有催化功能的抗体.至今已有50多个反应能用催化抗体来催化,对某些反应(如Diels-Alder反应),自然界不存在能催化它们的酶,也能用抗体来催化进行.因此,设计具有特异性的催化抗体,在化学、生物学、医学等领域无疑将显示巨大的应用潜力.茶普生(1)是一种非常重要的非甾体消炎镇痛药,其S构型的药效为R构型的38倍,研究用抗体催化水解萘普生乙酯(2)来拆分获得S构型的萘普生,具有重要的理论意义和潜在的应用价值.本文简要报道具有催化萘普生乙酯水解能力的多克隆抗体的研究.
One of the major discoveries in chemistry and immunology in the 1980s, the catalytic antibodies (abzymes), combined the extreme diversity of antibodies with the enormous catalysis capabilities of enzymes and others, opened the door to traditional chemistry and immunology research A new direction, the use of specially designed and synthesized organic molecules as a hapten, the carrier protein after the combination of immunization of animals and by cell hybridization technology, screening and isolation of monoclonal antibodies, successfully obtained with catalytic function of antibodies .Today More than 50 reactions have been catalyzed by catalytic antibodies. For some reactions (such as Diels-Alder reaction), there are no enzymes that can catalyze them in nature, but also can be catalyzed by antibodies. Therefore, Antibodies will undoubtedly show great potentials in the fields of chemistry, biology and medicine.Capsule (1) is a very important non-steroidal antiinflammatory and analgesic agent and its S-configuration is characterized by its R configuration 38 times, it is of great theoretical significance and potential application value to study the naproxen S structure obtained by the antibody hydrolysis of naproxen ethyl ester (2) .In this paper, water Research polyclonal antibodies capabilities.