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Afa家族串联重复序列因只出现在小麦及小麦族近缘属物种而得名,本研究从蒙古冰草中克隆得到一个Afa家族序列,长度为233 bp,命名为pAmAfa1,该序列在GENBANK中进行同源序列比对,结果表明该序列与大多数小麦族其他物种的Afa家族串联重复序列存在较高的相似性;系统进化分析表明,蒙古冰草pAmAfa1序列与大赖草pLrAfa3,pLrAfa5序列聚在一起,表明蒙古冰草P染色体组与大赖草的N、X染色体亲源关系较近。为了明确Afa家族串联重复序列在蒙古冰草染色体上的位置,双色荧光原位杂交技术被采用,以pAmAfa1为探针检测到杂交信号出现在染色体的末端或近端部的区域,每条染色体上都有杂交信号,表明Afa家族串联重复序列普遍存在于P染色体组中。
Afa family of tandem repeats, named only for the relatives belonging to wheat and Triticum, have been cloned from Agropyron mongolicum and have a length of 233 bp, named pAmAfa1, which was sequenced in GENBANK The results showed that there was a high similarity between this sequence and the Afa family tandem repeats of most Triticum species. Phylogenetic analysis showed that the sequence of pAmAfa1 was closely related to pLrAfa3 and pLrAfa5 Together, it shows that the P. pygmaceae P genome is closely related to the N and X chromosomes of Larvae. In order to confirm the location of Afa family tandem repeats on the chromosomes of Agropyron mongolicum, two-color fluorescence in situ hybridization was used. The hybridization signal was detected at the terminal or proximal part of chromosome by pAmAfa1 probe. On each chromosome There are hybridization signals, indicating that the Afa family of tandem repeats are prevalent in the P genome.