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目的了解消毒供应室环氧乙烷灭菌效果,防范干扰因素导致生物监测的假阳性结果,保证灭菌物品质量。方法应用快速型生物指示剂监测环氧乙烷灭菌效果,灭菌管和对照管快速阅读结果显示阳性的继续培养至48h,观察对照管和灭菌管的变色情况。结果共监测153锅次,4次快速监测结果为阳性,继续培养至48 h,结果与快速培养的结果不一致。结论消毒供应室在灭菌效果监测过程中要全面评判各种灭菌参数和监测结果,防范干扰因素导致的假阳性结果,快速生物监测后最好继续培养至48 h,以便能肉眼观察到有无细菌生长。
Objective To understand the sterilization effect of ethylene oxide in disinfection supply room and to prevent false positive results of biological monitoring caused by interference factors so as to ensure the quality of sterilized articles. Methods The rapid biological indicator was used to monitor the sterilizing effect of ethylene oxide. The results of rapid reading of the sterile tube and control tube showed that they were positive and continued to be cultured for 48 hours. The discoloration of the control tube and the sterile tube was observed. Results A total of 153 pots were monitored. Four fast monitoring results were positive and continued to 48 h. The results were inconsistent with the results of rapid culture. Conclusion The sterilizing supply room should evaluate various sterilization parameters and monitoring results comprehensively during the sterilization effect monitoring to prevent false positive results caused by interference factors. After rapid biological monitoring, it is better to continue culturing to 48 h so as to be able to observe with naked eye No bacterial growth.