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目的查明一起食源性疾病暴发的病原菌。方法按照国家标准检验方法 GB 4789—2008和WS 289—2008,对患者肛拭子以及外环境样本进行细菌分离培养、鉴定、毒力基因检测、血清学分型及脉冲场凝胶电泳分子分型。结果7份患者肛拭子中检出6株非O1群霍乱弧菌,4份外环境样本中检出1株非O1群霍乱弧菌,7株菌霍乱肠毒素Ctx AB基因检测结果均为阴性,脉冲场凝胶电泳分子分型显示患者分离株与外环境分离株分子带型同源。结论此次食源性疾病为非O1群霍乱弧菌污染外环境所致。PFGE分子分型方法对传染病暴发溯源起了重要作用。
Objective To identify pathogenic bacteria that erupt with foodborne illness. Methods According to the national standard test methods GB 4789-2008 and WS 289-2008, bacterial culture, identification, virulence gene test, serological typing and pulsed-field gel electrophoresis molecular typing were performed on the patients’ anal swabs and external environment samples. Results Six strains of V. cholerae O1 were detected in the anal swabs of seven patients. One strain of V. cholerae other than O1 was detected in four of the four environmental samples. The results of seven strains of Cholera enterotoxin Ctx AB gene were negative The molecular typing of pulsed-field gel electrophoresis showed that the isolates of patients and isolates had molecular band homology. Conclusion The foodborne disease is caused by non-O1 Vibrio cholerae polluting the environment. The PFGE molecular typing method plays an important role in the traceability of infectious diseases.