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A cultured female half-smooth tongue sole(C ynoglossus semilaevis) was crossed with a wild male, yielding the fi rst fi lial generation of pseudo-testcrossing from which 200 fi sh were randomly selected to locate the V ibrio anguillarum resistance trait in half-smooth tongue sole at its microsatellite linkage map. In total, 129 microsatellites were arrayed into 18 linkage groups, ≥4 each. The map reconstructed was 852.85 c M in length with an average spacing of 7.68 c M, covering 72.07% of that expected(1 183.35 cM). The V. anguillarum resistance trait was a composite rather than a unit trait, which was tentatively partitioned into Survival time in Hours After V. anguillarum Infection(SHAVI) and Immunity of V. A nguillarum Infection(IVAI). Above a logarithm of the odds(LOD) threshold of 2.5, 18 loci relative to SHAVI and 3 relative to IVAI were identifi ed. The 3 loci relative to IVAI explained 18.78%, 5.87% and 6.50% of the total phenotypic variation in immunity. The microsatellites bounding the 3 quantitative trait loci(QTLs) of IVAI may in future aid to the selection of V. anguillarum-immune half-smooth tongue sole varieties, and facilitate cloning the gene(s) controlling such immunity.
A cultured female half-smooth tongue sole (C ynoglossus semilaevis) was crossed with a wild male, yielding the fi rst fi lial generation of pseudo-test crossing from which 200 fi sh were randomly selected to locate the V ibrio anguillarum resistance trait in half- smooth tongue sole at its microsatellite linkage map. In total, 129 microsatellites were arrayed into 18 linkage groups, ≥ 4 each. The map reconstructed was 852.85 c M in length with an average spacing of 7.68 c M, covering 72.07% of that expected ( 1 183.35 cM). The V. anguillarum resistance trait was a composite rather than a unit trait, which was tentatively partitioned into Survival time in Hours After V. anguillarum Infection (SHAVI) and Immunity of V. A nguillarum Infection (IVAI). Above The 3 loci relative to IVAI were 18.78%, 5.87% and 6.50% of the total phenotypic variation in immunity. The logarithm of the odds (LOD) threshold of 2.5, 18 loci relative to SHAVI and 3 relative to IVAI were identified. microsatellites bounding the 3 quantitative trait loci (QTLs) of IVAI may in future aid to the selection of V. anguillarum-immune half-smooth tongue sole varieties, and facilitate cloning of the gene (s) controlling such immunity.