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目的:研究野马追药材中绿原酸含量测定的前提取方法及绿原酸含量的质控标准。方法:采用高效液相色谱法,比较了不同的提取方法、提取溶媒、提取时间和溶媒用量测定野马追药材中绿原酸含量。结果:采用超声提取方法提取野马追药材中绿原酸,且绿原酸高效液相法测定进样量在0.0908～1.362μg(r=0.9999)间呈良好的线性关系,平均回收率为100.12%,RSD为1.31%。结论:绿原酸超声提取方法和高效液相测定含量方法简便,可用于该药材的质量控制。
Objective: To study the extraction method of chlorogenic acid in wild horse chase herbs and the quality control standard of chlorogenic acid content. Methods: The HPLC method was used to compare the different extraction methods, extraction solvent, extraction time and the amount of solvent determination of wild horse chase herbs chlorogenic acid content. Results: The chlorogenic acid was extracted from the wild mustard herb by ultrasonic extraction, and the linear range was 0.0908 ~ 1.362μg (r = 0.9999) with the average recovery of 100.12% , RSD was 1.31%. Conclusion: The method of ultrasonic extraction of chlorogenic acid and high performance liquid are simple and convenient for quality control.