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采用RT-PCR和RACE方法,克隆了叶绿体ATP硫酸化酶基因的全长序列,命名为Ca ATPS1,Gen Bank登录号为KX009745。该基因包含1个长为1 377 bp的完整开放阅读框,编码458个氨基酸,预测分子量51.11 k D,等电点6.74。氨基酸同源性分析表明,该基因与Gen Bank中登录的茄科植物烟草、马铃薯及其它物种中的ATPS1氨基酸序列具有较高的同源性;进化树分析表明,辣椒Ca ATPS1与同为茄科属的烟草、马铃薯和胡麻属的芝麻处于同一进化分枝上,而与十字花科植物进化关系较远;荧光定量PCR分析显示,Ca ATPS1能被低温、干旱、高盐、机械损伤、过氧化氢、水杨酸、乙烯利以及DC3000侵染等各种胁迫和信号分子诱导。
The full-length sequence of chloroplast ATP sulfurylase gene was cloned by RT-PCR and RACE, named as Ca ATPS1, Gen Bank Accession No. KX009745. The gene contains a complete 1 377 bp open reading frame encoding 458 amino acids with a predicted molecular weight of 51.11 kD and an isoelectric point of 6.74. Amino acid homology analysis showed that this gene shared high homology with the ATPS1 amino acid sequence of Solanaceae tobacco, potato and other species registered in Gen Bank. Phylogenetic tree analysis showed that Ca ATPS1 was homologous to solanaceae Genus tobacco, potato and flax genus sesame belong to the same branch of evolution, while cruciferous plants evolved more distantly. Fluorescence quantitative PCR analysis showed that Ca ATPS1 could be damaged by low temperature, drought, high salt, mechanical damage and peroxidation Hydrogen, salicylic acid, ethephon and DC3000 infection and other stress and signal molecule induction.