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为探讨二脱氧肌苷(ddI)对培养的背根神经节(DRG)神经元的影响,我们对分散培养的胎鼠DRG神经元培养3d后,再分别以不同浓度的ddI(1μg/ml,5μg/ml,10μg/mll和20μg/ml)孵育3d。终止培养后,行微管相关蛋白2(MAP2)标记,用共聚焦激光扫描显微镜观察神经元胞体和突起的改变。结果表明,DRG神经元用ddI孵育3d,神经元突起的数目减少和长度变短,呈剂量依赖性,而神经元的直径则没有变化。本研究的结果表明,ddI可影响培养的DRG神经元突起的再生和生长。
To investigate the effect of dideoxyinosine (ddI) on cultured dorsal root ganglion (DRG) neurons, the cultured DRG neurons were cultured for 3 days and then treated with different concentrations of ddI (1μg / ml, 5 μg / ml, 10 μg / ml and 20 μg / ml) for 3 days. After termination of culture, the microtubule-associated protein 2 (MAP2) marker was labeled and changes in neuronal somatic cells and protrusions were observed with a confocal laser scanning microscope. The results showed that DRG neurons incubated with ddI for 3d, the number of neurons decreased and the length became shorter, in a dose-dependent manner, while the diameter of neurons did not change. The results of this study indicate that ddI can affect the regeneration and growth of neurite outgrowths in cultured DRG neurons.