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目的:研究5-烯丙基-7-二氟甲氧基白杨素(AFMC)选择性增强肿瘤坏死因子凋亡诱导配体(TRAIL)对人肺癌A549细胞毒性作用。方法:体外培养人肺癌A549细胞和人胚肺WI-38细胞。全自动生化分析仪测定细胞上清液乳酸脱氢酶活性。碘化丙啶(PI)染色流式细胞术(FCM)分析细胞凋亡率。结果:AFMC与TRAIL合用协同性增强A549细胞毒性,合并用药效应指数(CI)值为0.019。亚细胞毒浓度AFMC与TRAIL合用对人胚肺WI-38无明显细胞毒性。亚细胞毒浓度AFMC与TRAIL合用诱导A549细胞凋亡率为37.80%±1.78%,而WI-38细胞凋亡率为0.35%±0.12%。结论:亚毒性浓度的AFMC具有选择性增强TRAIL对人肺癌A549细胞毒作用。
AIM: To investigate the cytotoxicity of 5-allyl-7-difluoromethoxyn-chrysene (AFMC) on human lung cancer cell line A549 selectively enhanced apoptosis-inducing ligand (TRAIL). Methods: Human lung adenocarcinoma A549 cells and human embryo lung WI-38 cells were cultured in vitro. Automatic biochemical analyzer for determination of cell supernatant lactate dehydrogenase activity. Propidium iodide (PI) staining flow cytometry (FCM) analysis of apoptosis rate. Results: The combination of AFMC and TRAIL synergistically enhanced the cytotoxicity of A549 cells. The combined drug effect index (CI) was 0.019. Subcellular cytotoxicity of AFMC in combination with TRAIL did not show cytotoxicity on human embryo lung WI-38 cells. The apoptosis rate of A549 cells induced by sub-cytotoxicity of AFMC and TRAIL was 37.80% ± 1.78%, while the apoptosis rate of WI-38 cells was 0.35% ± 0.12%. Conclusion: AFMC with sub-toxic concentration can selectively enhance the cytotoxic effect of TRAIL on human lung cancer A549 cells.