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目的分析三甲基氯化锡(TMT)低剂量染毒小鼠血清、肝脏和脑的氧化损伤情况方法将56只雄性昆明种小鼠分为7组,每组8只。2个实验组分别腹腔注射TMT 1.0和2.15 mg/kg,对照组给予无菌生理盐水,在染毒后24h处死,测定血清、肝脏和脑组织中的SOD和MDA水平;另外2个实验组腹腔注射TMT 2.15 mg/kg,2个对照组给予无菌生理盐水,分别于染毒后3和7 d处死,测定血清、肝脏和脑中的SOD和MDA水平。结果 2.15 mg/kg TMT染毒组动物在20 h后开始出现神经毒性症状,2.15 mg/kg TMT组动物染毒后24h,血清、肝脏和脑组织中MDA水平较对照组明显增加,肝脏SOD明显下降(P<0.01或P<0.05),1.0 mg/kg TMT染毒后24 h,血清和脑组织中MDA较对照组明显增加(P<0.01或P<0.05),2.15 mg/kg TMT染毒后3 d肝脏、脑组织SOD较对照组明显下降(P<0.01),染毒7 d,血清MDA增高,肝脏SOD水平明显下降(P<0.05)。结论给予小鼠低剂量TMT染毒小鼠24 h后,血清、肝脏和脑组织均会造成一定程度的过氧化损伤,而在染毒后3和7 d肝脏和脑中SOD会发生损耗,而小鼠过氧化损伤程度进一步缓解。
Objective To analyze the oxidative damage of serum, liver and brain in mice exposed to low doses of trimethyltin chloride (TMT) Methods Fifty-six male Kunming mice were divided into 7 groups with 8 mice in each group. Two experimental groups were intraperitoneally injected with TMT 1.0 and 2.15 mg / kg respectively. The control group was given sterile saline and killed at 24h after exposure. The levels of SOD and MDA in serum, liver and brain tissue were measured. The other two groups TMT 2.15 mg / kg was injected, and two control groups were given sterile saline and sacrificed at 3 and 7 d after exposure respectively. The levels of SOD and MDA in serum, liver and brain were measured. Results The symptoms of neurotoxicity began to appear in TMT group at 2.15 mg / kg after 20 h. The levels of MDA in serum, liver and brain tissue at 24 h after exposure to 2.15 mg / kg TMT group were significantly increased compared with the control group (P <0.01 or P <0.05). MDA in serum and brain tissue increased significantly (P <0.01 or P <0.05) and TMT at 2.15 mg / kg 24 h after 1.0 mg / kg TMT exposure After 3 days, SOD in liver and brain tissues decreased significantly (P <0.01). After 7 days of exposure, serum MDA increased and SOD level in liver decreased significantly (P <0.05). Conclusion The serum, liver and brain tissues of mice treated with low dose of TMT for 24 h could cause some degree of peroxidation injury, while the loss of SOD in liver and brain at 3 and 7 days after exposure The degree of peroxide damage in mice was further alleviated.