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硫癸醇(1,10-二羟乙基硫代癸烷)是一种广泛用于治疗血胆固醇和血脂蛋白过多的降血脂药.它在体内的药物浓度测定,以往文献未见报道.本文首次采用GC法测定硫癸醇在人血浆中的浓度,其优点为灵敏度高,专属性好.实验方法:(1)提取与衍生化:取1ml血浆于15ml离心管中,加6ml氯仿和100μl甲醇(含1μg/ml的1,8-二羟乙基硫代辛烷,内标液).振摇5min,离心5min.取有机层5.9ml移到10ml的锥形试管中,真
Thiodecanol (1,10-dihydroxyethylthiodecane) is a lipid-lowering drug widely used in the treatment of hypercholesterolemia and hyperlipidemia, and it has not been previously reported in the literature for the determination of its drug concentration in the body. In this paper, the first determination of the concentration of thio-decanol in human plasma by GC has the advantages of high sensitivity and good specificity.Experimental methods: (1) Extraction and derivatization: Take 1 ml of plasma in a 15 ml centrifuge tube, add 6 ml of chloroform and 100μl methanol (containing 1μg / ml of 1,8-dihydroxyethylthiooctane, internal standard solution) Shaken 5min, centrifuged 5min. Take 5.9ml organic layer was transferred to a 10ml conical tube, really