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目的采用体外实验评价溴系阻燃剂六溴环十二烷(HBCDs)对人乳腺(HBL-100)细胞的毒性,并探索其可能的致毒机制。方法将HBL-100细胞暴露于0(对照)、5、10、20、50、100 mg/L的HBCDs。暴露2 h后,检测上清液活性氧(ROS)含量;暴露24 h后,检测细胞相对增殖率、胞内及上清液乳酸脱氢酶(LDH)活力和上清液中丙二醛(MDA)含量。结果与对照组比较,仅50~100 mg/L HBCDs暴露HBL-100细胞的增殖率明显较低,差异均有统计学意义(P<0.05);其他浓度组HBL-100细胞的增殖率变化不显著。各浓度HBCDs暴露组HBL-100细胞内LDH的漏出率均较高,5~50 mg/L HBCDs暴露组HBL-100细胞上清液中MDA和ROS的含量也较高,差异均有统计学意义(P<0.05,P<0.01);且随着HBCDs暴露浓度的升高,HBL-100细胞内LDH的漏出率呈升高趋势,上清液中MDA和ROS的含量均呈先增加后减少的趋势。结论较高浓度HBCDs具有细胞毒性;低浓度HBCDs导致细胞氧化损伤,而高浓度HBCDs破坏细胞防御系统,导致细胞直接死亡。
Objective To evaluate the toxicity of brominated flame retardant HBCDs on human breast (HBL-100) cells in vitro and to explore its possible mechanisms of toxicity. Methods HBL-100 cells were exposed to 0 (control), 5, 10, 20, 50, 100 mg / L HBCDs. After exposure for 2 h, the content of reactive oxygen species (ROS) in the supernatant was measured. After exposure for 24 h, the relative cell proliferation rate, intracellular and supernatant lactate dehydrogenase (LDH) activity and the malondialdehyde MDA) content. Results Compared with the control group, the proliferation rate of HBL-100 cells exposed to 50-100 mg / L HBCDs was significantly lower than that of the control group (all P <0.05). The proliferation rate of HBL-100 cells in other concentrations group was not changed Significant. The leakage rates of LDH in HBL-100 cells were higher in each concentration of HBCDs exposed group, and the contents of MDA and ROS in HBL-100 cells supernatant were also higher in 5 ~ 50 mg / L HBCDs exposed group, the differences were statistically significant (P <0.05, P <0.01). With the increase of HBCDs exposure, the leakage rate of LDH in HBL-100 cells increased and the content of MDA and ROS in the supernatant increased first and then decreased trend. Conclusions Higher concentration of HBCDs is cytotoxic. Low concentrations of HBCDs lead to cell oxidative damage, while high concentrations of HBCDs destroy the cell defense system, resulting in direct cell death.