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猪眼晶状体以不同时间(12～96小时)进行培养,在每次培养的最后6小时用~3H-亮氨酸掺入蛋白质中,提取水溶性蛋白,用凝胶过滤分离晶体蛋白,以~3H-亮氨酸掺入量表示蛋白质合成速度。晶状体在含小牛血清的199培养液中,培养在12小时之内,晶状体合成蛋白质的速度最高,24小时后其速度下降40％,48小时后其速度下降趋于稳定,并保持在一个较低的水平上。在所合成的蛋白质中以α-晶体蛋白合成能力最强,除合成晶体蛋白外,还合成大量小分子肽,这些可能是晶体蛋白的片段,它们的合成速度尤为显著。
Porcine eye lenses were cultured at different times (12 to 96 hours), spiked with ~ 3H-leucine in the final 6 hours of each culture, water-soluble proteins were extracted, and the crystal proteins were separated by gel filtration, 3H-Leucine incorporation indicates protein synthesis rate. After 12 hours of incubation, the lens produced the highest rate of protein synthesis in 40% fetal bovine serum-containing medium, and its rate of decrease was 40% after 24 hours. After 48 hours, the rate of decrease of the lens tended to be stable and maintained at a higher level Low level. Among the synthesized proteins, α-crystallin has the strongest ability to synthesize. In addition to synthesizing crystal proteins, a large number of small-molecule peptides are synthesized, which may be fragments of crystal proteins, and their synthesis speed is particularly remarkable.