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本文分析丝裂霉素C(MMC)和造纸厂流出物对H.fossilis鱼外周血红细胞中微核的影响,用以估价鱼外周血涂片的微核试验对环境遗传毒物的生物学监测是否适用,和用脊椎动物系统检测造纸厂流出物的可能的遗传毒效应。实验用H.fossilis鱼(一种极普通的印度猫鱼)。这种鱼生命力强,能在实验室容量有限的鱼缸中存活数月。将在自来水中适应一周的体重15~20 g活泼的鱼随机分到各组。取Orient造纸厂各工段(包括成浆、漂白、染色等)流出物的混合物作受试物。第一组实验:给32条鱼经腹腔注射MMC水溶液,MMC剂量分别为0.0、0.5、1.0和
This article analyzes the effect of mitomycin C (MMC) and paper mill effluents on the micronuclei in peripheral blood red blood cells of H. fossilis fish to assess the biological monitoring of environmental genetic toxicants in micronucleus tests of fish peripheral blood smear whether Applicable, and probable genotoxic effects of vertebrate systems for the detection of paper mill effluents. Experiment with H.fossilis fish (a very common Indian catfish). This fish is very viable and can survive for months in aquariums with limited lab capacity. In the tap water to adapt to a week of weight 15 ~ 20 g lively fish were randomly assigned to each group. Take the Orient paper mill sections (including pulp, bleach, dyeing, etc.) mixture of the effluent as a test substance. The first group of experiments: 32 fish were injected intraperitoneally MMC solution, MMC dose were 0.0,0.5,1.0 and