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研究目的探讨反复呼吸道感染（ＲＲＴＩ）患儿血清微量元素含量，外周血Ｔ细胞亚群的变化及其意义。研究方法ＲＲＴＩ患儿９２例，男５８例，女３４例。４２名健康儿童为对照组。取清晨（７时～９时）空腹静脉血（肝素抗凝）（３～５）ｍｌ。血清微量元素谱测定采用Ｓｐｅｃｔｒａｓｐａｎ－Ｖ型三电极等离子原子发射直读光谱仪（ＤＣＰ－ＡＥＳ），同时测定１２种微量元素含量，如锌（７ｎ）、锰（Ｍｎ）、铝（Ａｌ）、镉（Ｃｄ）、铁（Ｆｅ）、铅（Ｐｂ）、铜（Ｃｕ）、镍（Ｎｉ）、钛（Ｔｉ）、钴（Ｃｏ）、锂（Ｌｉ）、铬（Ｃｒ）等。Ｔ细胞正群测定采用抗人单克隆抗体致敏红细胞花环法，Ｔ细胞增殖功能测定采用３Ｈ－ＴｄＲ掺入法。结果ＲＲＴＩ患儿血清微量元素Ｚｎ、Ｐｂ、Ｎｉ、Ｃｏ、Ｌｉ、Ｆｅ、Ｔｉ明显低于正常对照组。外周血Ｔ细胞亚群、细胞百分率、比值及Ｔ细胞增殖功能显著低于正常对照组，血清ＩｇＧ也低于对照组。
Objective To investigate the changes of serum microelement content and T lymphocyte subsets in children with recurrent respiratory tract infection (RRTI) and its significance. Research Methods 92 cases of RRTI children, 58 males and 34 females. Forty-two healthy children served as control group. Take early morning (7:00 ~ 9:00) fasting venous blood (heparin anticoagulant) (3 ~ 5) ml. Serum trace element spectra were measured using a Spectraspan-V three-electrode plasma atomic emission spectrometer (DCP-AES) with simultaneous determination of 12 trace elements such as zinc (7n), manganese (Mn), aluminum (Al) Cd, Fe, Pb, Cu, Ni, Ti, Co, Li, Cr and the like. T cell positive group assay using anti-human monoclonal antibody sensitized erythrocyte rosette method, T cell proliferation assay using 3H-TdR incorporation. Results Serum trace elements Zn, Pb, Ni, Co, Li, Fe and Ti in RRTI children were significantly lower than those in normal controls. Peripheral blood T cell subsets, cell percentage, ratio and T cell proliferation was significantly lower than the normal control group, serum IgG was lower than the control group.