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以拉曼被孢霉（Mortierellaramanniana）SM541为原始菌株，经过紫外线复合氯化锂诱变处理，得到突变株SM541－9，其生物量由126g／L提高到28．8g／L，油脂含量由5．8g／L提高到15.7g／L。花生四烯酸含量由321mg／L增加到623mg／L。传代实验表明，SM541－9具有良好的遗传稳定性，以葡萄糖为碳源，硝酸钾为氮源的最适培养条件下，10L罐发酵，生物量和花生四烯酸含量较原始菌株分别提高了45．2％和109.5％。在液体发酵过程中，菌丝老化、增大溶氧量、间歇流加葡萄糖都有利于花生四烯酸的合成。
The mutant strain SM541-9 was obtained by mutagenesis of UV-combined lithium chloride with the original strain Mortierellaramanniana SM541, its biomass increased from 126g / L to 28.8g / L, and the oil content increased from 5 .8g / L increased to 15.7g / L. Arachidonic acid increased from 321mg / L to 623mg / L. The passage experiment showed that SM541-9 had good genetic stability. The fermentation of 10L pot with the optimum conditions of glucose as carbon source and potassium nitrate as nitrogen source increased biomass and arachidonic acid content compared with the original strain 45.2% and 109.5%. In the liquid fermentation process, mycelium aging, increasing the amount of dissolved oxygen, intermittent flow plus glucose are conducive to arachidonic acid synthesis.