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目的了解甘氨酰谷氨酰胺二肽(简称甘谷二肽)对严重烧伤大鼠心肌力学功能的保护作用,并探讨其机制。方法将136只Wistar大鼠分成对照组8只(不予烧伤)、烧伤组32只、谷氨酰胺组32只、甘氨酸组32只、甘谷二肽组32只。后4组均造成30%TBSAⅢ度烧伤。5组大鼠依次给予酪氨酸1.5g·kg~(-1)·d~(-1)、酪氨酸1.5 g·kg~(-1)·d~(-1)、谷氨酰胺1.0 g·kg~(-1)·d~(-1)+酪氨酸0.5 g·kg~(-1)·d~(-1)、甘氨酸0.5 g·kg~(-1)·d~(-1)+酪氨酸1.0g·kg~(-1)·d~(-1)、甘谷二肽1.5 g·kg~(-1)·d~(-1)。伤后12、24、48、72 h检测大鼠心肌组织还原型谷胱甘肽(GSH)、腺苷一磷酸(AMP)、腺苷二磷酸(ADP)、腺苷三磷酸(ATP)、细胞能荷(EC)的含量和主动脉收缩压(AOSP)、主动脉舒张压(AODP)及左心室收缩压(LVSP)、左心室压力变化最大上升速率(+dp/dtmax)。结果后4组大鼠伤后心肌组织GSH、ATP和EC含量以及AOSP、AODP、LVSP、+dp/dtmax检测值普遍低于对照组(P<0.01),AMP、ADP含量则普遍高于对照组(P<0.01)。与烧伤组比较,后3组上述指标均有明显改变。伤后12h甘谷二肽组的GSH含量为(72.7±1.7)μmol/g,高于谷氨酰胺组(67.8±3.8)μmol/g(P<0.01);该时相点EC、AOSP的检测值亦高于谷氨酰胺组(P<0.01)。伤后48 h甘谷二肽组GSH、EC、AOSP的检测值高于甘氨酸组(P<0.01)。结论大鼠烧伤后给予谷氨酰胺、甘氨酸和甘谷二肽,能不同程度改善心肌组织GSH、高能磷酸化合物含量,缓解心肌力学功能的下降。甘谷二肽的疗效明显优于谷氨酰胺或甘氨酸,提示它对烧伤后心脏功能的保护作用具有协同效应。
Objective To understand the protective effect of glycylglycine dipeptide (Gan glutathione dipeptide) on myocardial mechanical function in severely burned rats and its mechanism. Methods 136 Wistar rats were divided into control group (no burn), burn group (32), glutamine group (32), glycine group (32) and glutamyl dipeptide group (32) After 4 groups were caused by 30% TBSA degree of burn. The rats in the 5 groups were given 1.5 g · kg -1 d · tyr and 1.5 g · kg -1 d -1 of tyrosine, g · kg -1 · d -1 · tyrosine 0.5 g · kg -1 · d -1 and glycine 0.5 g · kg -1 · d -1 -1) + tyrosine 1.0 g · kg -1 · d -1 and gluten dipeptide 1.5 g · kg -1 · d -1. At 12, 24, 48 and 72 h after injury, the contents of glutathione (GSH), adenosine monophosphate (AMP), adenosine diphosphate (ADP), adenosine triphosphate (AOSP), aortic pressure (AODP) and left ventricular systolic pressure (LVSP), and the maximum rate of increase of left ventricular pressure (+ dp / dtmax). Results The levels of GSH, ATP and EC in myocardial tissue and the levels of AOSP, AODP, LVSP and + dp / dtmax were all lower in the 4 groups than those in the control group (P <0.01), while the contents of AMP and ADP were generally higher than those in the control group (P <0.01). Compared with the burn group, after the above three groups of indicators have significantly changed. The glutathione dipeptide group’s GSH content was (72.7 ± 1.7) μmol / g at 12h after injury, which was higher than that of glutamine group (67.8 ± 3.8μmol / g) (P <0.01) Also higher than glutamine group (P <0.01). Glucagon dipeptide group GSH, EC, AOSP 48 h after injury was higher than the glycine group (P <0.01). CONCLUSION: Glutamine, glycine and glutathione dipeptide were administered to rats after burn injury, which could improve the content of GSH and high-energy phosphate compounds in myocardium and relieve the decrease of myocardial mechanics function. Gan glutathione dipeptide significantly better than glutamine or glycine, suggesting that it has a synergistic effect on the protection of cardiac function after burn.