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目的研究原发性肝癌(primary hepatocarcinoma,PHC)组织中肿瘤相关巨噬细胞(tumor-associated macrophage,TAM)、肥大细胞(mast cells,MC)和微血管(microvessel,MV)的计数及其临床病理意义,并探讨了三者之间的相互关系.方法应用ABC免疫组化法,对47例PHC手术切除标本常规石蜡包埋切片分别检测TAM、MC和MV,并高倍镜下计数.结果47例PHC癌组织中TAM、MC和MV计数均显著高于癌旁组织(TAM:70.27±17.93个/HP vs.44.15±9.10个/HP,P<0.01;MC:15.46±4.54个/HP vs.8.08±2.03个/HP,P<0.01;MV:67.30±13.68个/HP vs.37.20±10.58个/HP,P<0.01);TAM与MV在术前AFU(α-岩藻糖)小于等于10μg/L病例的计数显著高于AFU大于10μg/L病例的计数(TAM:74.13±18.33个/HP vs.61.15±13.54个/HP,P<0.05;MV:70.41±13.03个/HP vs.59.97±12.69个/HP,P<0.05);伴转移病例癌组织中的MV计数(73.50±13.77个/HP)显著高于无转移病例(64.10±12.68个/HP,P<0.05);MC、TAM、MV在PHC中的计数与PHC其他主要临床病理特征之间均无明显关系;癌组织中TAM计数与MV计数呈正相关(P<0.01,r=0.686),MC计数与MV计数呈正相关(P<0.05,r=0.348).结论MC、TAM、MV的计数与PHC的发生、发展关系密切;MV计数高的PHC易于发生浸润和转移;TAM、MC与PHC的血管生成可能有密切的关系.
Objective To study the counting of tumor-associated macrophage (TAM), mast cells (MC) and microvessel (MV) in primary hepatocarcinoma (PHC) and their clinicopathological significance , And explored the relationship among the three.Methods ABC immunohistochemical method was used to detect TAM, MC and MV in 47 cases of PHC surgically removed paraffin embedded sections and counted under high magnification.Results 47 cases of PHC TAM, MC and MV counts in cancer tissues were significantly higher than those in adjacent tissues (TAM: 70.27 ± 17.93 / HP vs.44.15 ± 9.10 / HP, P <0.01; MC:15.46 ± 4.54 / HP vs.8.08 ± 2.03 cells / HP, P <0.01; MV: 67.30 ± 13.68 cells / HP vs 3.7.20 ± 10.58 cells / HP, P <0.01); TAM and MV were less than or equal to 10 μg / L The count of cases was significantly higher than the counts of AFU> 10 μg / L cases (TAM: 74.13 ± 18.33 / HP vs.61.15 ± 13.54 /HP, P<0.05;MV:70.41 ± 13.03 / HP vs.59.97 ± 12.69 /HP, P <0.05). The count of MVs in cancerous tissues with metastasis (73.50 ± 13.77 / HP) was significantly higher than that in non-metastatic cases (64.10 ± 12.68 / Hp, P <0.05) The counts in PHC are related to other major clinical pathologies of PHC (P <0.01, r = 0.686), and there was a positive correlation between MC count and MV count (P <0.05, r = 0.348) .Conclusion The expression of MC, TAM, MV counts are closely related to the occurrence and development of PHC; PHC with high MV counts is prone to infiltrate and metastasize; TAM, MC and angiogenesis of PHC may be closely related.