20 0 0年夏季，我们采用牛粪锯末堆料发酵技术制双孢菇菌种。制母种 2 3 0 0瓶，成功 2 2 98瓶，成功率99 9%；制原种 2 80 0 0瓶，成功率 98 1 %；制栽培种51 70 0 0瓶，成功率 93 2 %。现将制种方法介绍如下，供广大菇友参考。1　备　　料　①锯末：选未变质锯末过筛后在阳 In the summer of 20o0, we used Agaricus sativus as a starter to produce Agaricus bisporus. There were 2300 bottles of mother sage and 2298 bottles successfully, with a success rate of 99.9%. There were 2 80 0 0 bottles of the original system, with a success rate of 98.1%. 51 70 0 0 bottles were cultivated with a success rate of 93.2% . Seed production methods are introduced below, for the majority of mushroom friends reference. 1 preparation ① Sawdust: selected non-destructive sawdust after screening in the sun