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目的建立高效液相色谱波长切换技术同时测定尤力克柠檬叶中绿原酸、圣草次苷、芦丁、地奥司明4种活性成分含量的方法。方法使用Sepax Amethyst C18-H柱(250 mm×4.60 mm,5μm),以乙腈(A)-0.1%甲酸水溶液(B)为流动相进行梯度洗脱,流速1.0 m L·min-1。检测波长为324 nm(0~20 min,绿原酸),284 nm(20~68 min,圣草次苷),256 nm(68~80 min,芦丁),346 nm(80~125 min,地奥司明)。柱温25℃,进样量20μL。结果 4种化合物在测定的范围内峰面积与浓度之间均表现出良好的线性关系,平均加样回收率分别为98.55%(RSD=1.75%)、98.34%(RSD=2.36%)、101.41%(RSD=1.91%)、99.83%(RSD=2.08%)。结论该方法简便、准确,重复性好,可用于尤力克柠檬叶的质量控制。
OBJECTIVE To establish a HPLC method for the simultaneous determination of four active ingredients of chlorogenic acid, eriocitrin, rutin and desoacetamide in lemon leaves of Youlicher. The method was performed on a Sepax Amethyst C18-H column (250 mm × 4.60 mm, 5 μm) using a mobile phase of acetonitrile (A) -0.1% formic acid (B) as the mobile phase at a flow rate of 1.0 mL · min -1. The detection wavelength was 324 nm (0-20 min, chlorogenic acid), 284 nm (20-68 min, eriocitrin), 256 nm (68-80 min, rutin), 346 nm Di Austrian Ming). Column temperature 25 ℃, injection volume 20μL. Results The four compounds showed a good linear relationship between peak area and concentration in the range of determination. The average recoveries were 98.55% (RSD = 1.75%), 98.34% (RSD = 2.36%), 101.41% (RSD = 1.91%), 99.83% (RSD = 2.08%). Conclusion The method is simple, accurate and reproducible. It can be used for the quality control of Yurik lemon leaves.