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目的:探讨人参皂苷Rg1对脂多糖(LPS)诱导心肌细胞肥大的保护作用及机制。方法:利用体外培养模型,以脂多糖1 mg/L诱导心肌细胞肥大,观察不同剂量人参皂苷Rg1(12.5、25、50μmol/L)及环孢菌素A(CsA)对脂多糖诱导的心肌肥大的抑制作用。用考马斯亮蓝法测心肌细胞蛋白质含量;消化分离法及计算机图像分析系统检测心肌细胞体积;倒置相差显微镜下观察细胞形态大小;采用Fluo-3/Am荧光染色剂负载细胞,在激光共聚焦显微镜下测定细胞内钙离子浓度的瞬间变化;电泳法测定钙调磷酸酶(CaN)的表达。结果:与正常对照组相比,脂多糖模型组使心肌细胞总蛋白质含量增加,细胞体积增大,心肌细胞内[Ca2+]i瞬间峰值增大,钙调磷酸酶表达增加。与模型组相比,人参皂苷Rg1(25、50μmol/L)可有效改善脂多糖诱导的心肌细胞肥大,抑制心肌细胞内[Ca2+]i瞬间峰值增大,抑制钙调磷酸酶表达增加。结论:人参皂苷Rg1可通过抑制Ca2+/钙调磷酸酶信号通路抑制脂多糖诱导的心肌细胞肥大。
Objective: To investigate the protective effect and mechanism of ginsenoside Rg1 on cardiomyocyte hypertrophy induced by lipopolysaccharide (LPS). Methods: Cardiomyocyte hypertrophy was induced by lipopolysaccharide (1 mg / L) in vitro and the effects of different dosages of ginsenoside Rg1 (12.5,25,50μmol / L) and cyclosporin A (CsA) on lipopolysaccharide-induced cardiac hypertrophy Inhibition. The protein content of cardiomyocytes was measured by Coomassie brilliant blue method. The volume of cardiomyocytes was detected by digestion and separation method and computer image analysis system. The morphology of cells was observed under inverted phase contrast microscope. The cells were loaded with Fluo-3 / Am fluorescent dye, The transient changes of intracellular calcium concentration were measured. The expression of calcineurin (CaN) was determined by electrophoresis. Results: Compared with the normal control group, the lipopolysaccharide model group increased the total protein content of cardiomyocytes, the cell volume increased, the peak of [Ca2 +] i in the myocardial cells increased and the expression of calcineurin increased. Compared with the model group, ginsenoside Rg1 (25,50μmol / L) could effectively improve LPS-induced cardiomyocyte hypertrophy, inhibit the peak of [Ca2 +] i in myocardium and increase the expression of calcineurin. Conclusion: Ginsenoside Rg1 can inhibit LPS-induced cardiomyocyte hypertrophy by inhibiting the Ca2 + / calcineurin signaling pathway.