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利用胆红素溶液在光照下易氧化变色及其吸收光谱发生显著变化的性质,在波长453nm处,测其光照前后吸收度差值,该差值与胆红素溶液浓度呈线性
Using bilirubin solution in the light of easy oxidation and discoloration of the absorption spectrum of the nature of significant changes in the wavelength of 453nm, measured before and after the light absorption difference, the difference and the concentration of bilirubin solution was linear