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AIM: To study the effect of hydrogen sulfide(H2S) on severe acute pancreatitis(SAP) in a rat model.METHODS: Sprague-Dawley(SD) rats were administered an intraperitoneal injection of saline containing 20% L-Arg(250 mg/100 g) hourly for over 2 h to induceSAP. The rats were treated with DL-propargylglycine(PAG, 50 mg/kg) or different dosages of Na HS(5 mg/kg, 10 mg/kg, 20 mg/kg or 100 mg/kg). PAG or Na HS was administered 1 h before induction of pancreatitis. Rats were sacrificed 24 h after the last L-Arg injection. Blood and pancreas tissues were collected.RESULTS: The H2 S and cystathionine-γ-lyase m RNA levels in SAP rats were significantly lower than those in the control group, and treatment with PAG further reduced the H2 S level. Nevertheless, H2 S was significantly increased after Na HS administration compared with the SAP group, and the degree of upregulation was associated with the Na HS dosage. Na HS reduced the levels of plasma amylase, interleukin-6 and myeloperoxidase in pancreatic tissue. Na HS suppressed the degradation of Ik Bα and the activity of nuclear factor-k B, as well as the phosphorylation of PI3K/AKT.CONCLUSION: H2 S plays an anti-inflammatory role in SAP in vivo.
AIM: To study the effect of hydrogen sulfide (H2S) on severe acute pancreatitis (SAP) in a rat model. METHODS: Sprague-Dawley (SD) rats were administered an intraperitoneal injection of saline containing 20% L-Arg 100 g) hourly for over 2 h to induce SAP. The rats were treated with DL-propargylglycine (PAG, 50 mg / kg) or different dosages of Na HS (5 mg / kg, 10 mg / kg, 20 mg / kg or 100 mg / kg). PAG or Na HS was administered 1 h before induction of pancreatitis. Rats were sacrificed 24 h after the last L-Arg injection. Blood and pancreas tissues were collected .RESULTS: The H2 S and cystathionine-γ-lyase m RNA, in SAP rats were significantly lower than those in the control group, and treatment with PAG further reduced the H2 S level. Nevertheless, H2 S was significantly increased after Na HS administration compared with the SAP group, and the degree of upregulation was associated with the HS HS dosage. Na HS reduced the levels of plasma amylase, interleukin-6 and myeloperoxidase in pan creatic tissue. Na HS suppressed the degradation of Ik Bα and the activity of nuclear factor-k B, as well as the phosphorylation of PI3K / AKT.CONCLUSION: H2 S plays an anti-inflammatory role in SAP in vivo.