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目的观察成年SD大鼠阴茎包皮和包皮系带内神经型一氧化氮合酶(nNOS)免疫阳性神经末梢的分布和起源。方法免疫组织化学法观察nNOS免疫阳性神经末梢的分布,荧光金(Fluoro-gold,FG)逆行追踪和nNOS免疫荧光标记相结合法研究大鼠包皮系带内nNOS免疫阳性神经末梢的起源。结果成年SD大鼠阴茎包皮和包皮系带内均有nNOS免疫阳性神经末梢存在,这些神经末梢主要位于表皮基底层和真皮乳头层,呈树枝状或念珠状分布。阴茎系带处nNOS免疫阳性神经末梢的图像光密度值(3.4±0.38)明显大于阴茎包皮处(2.2±0.45)。FG逆标阳性细胞位于大鼠第六腰髓对应的背根神经节(L6-DRG)(9.6±1.2)个/mm2和第一骶髓对应的背根神经节(S1-DRG)(1.2±0.2个/mm2)内。阳性细胞大中小不等,大多沿神经束成行排列或散在分布。nNOS免疫荧光标记细胞在L6-DRG和S1-DRG内分别为(24.2±2.6)个/mm2和(24.1±2.1)个/mm2,细胞大多呈中小型。FG/nNOS双标阳性细胞均为中小型,其数量接近FG逆标阳性细胞总数的一半。结论大鼠阴茎包皮系带内含有浓密的nNOS免疫阳性神经末梢,这些神经末梢源自于L6-DRG和S1-DRG。
Objective To investigate the distribution and origin of neuronal nitric oxide synthase (nNOS) immunoreactive nerve terminals in the penile foreskin and foreskin of adult SD rats. Methods Immunohistochemistry was used to observe the distribution of nNOS immunoreactive nerve endings. Fluoro-gold (FG) retrograde tracing and nNOS immunofluorescence labeling were used to study the origin of nNOS immunoreactive nerve terminals in rat foreskin. Results The nNOS immunoreactive nerve terminals were found in the penile foreskin and foreskin of adult SD rats. These nerve terminals mainly located in the basal and dermal papilla layers of the epidermis, showing dendritic or rosary distribution. The optical density value of nNOS immunoreactive nerve terminals at penile ligament (3.4 ± 0.38) was significantly larger than that of penile foreskin (2.2 ± 0.45). FG reverse positive cells were located in the dorsal root ganglion (L6-DRG) (9.6 ± 1.2) / mm2 corresponding to the sixth spinal cord of rat and S1-DRG corresponding to the first sacral canal (1.2 ± 0.2 / mm2). Large and small positive cells ranging, mostly arranged along the bundle of nerve fibers or scattered distribution. The number of nNOS immunofluorescent labeled cells was (24.2 ± 2.6) / mm2 and (24.1 ± 2.1) / mm2 in L6-DRG and S1-DRG, respectively. Both FG / nNOS positive double positive cells were small and medium in size, nearly half the number of positive FG positive cells. Conclusion The rat penile foreskin ligament contains dense nNOS immunoreactive nerve endings derived from L6-DRG and S1-DRG.