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Testis-specific protease 50(TSP50) has been identified as a testis-specific protein that is expressed abnormally in most human breast cancer samples,which makes it an attractive molecular marker and a potential target for diagnosis and therapy.In the present study,we prepared a panel of monoclonal antibodies(mAbs) with high specificity and sensitivity against TSP50 by hybridoma method and characterized them by ELISA,Western blot,immunofluroescence and immunohistochemical analyses.The results show that all of the 9 different clones can specifically bind to TSP50.The mAbs against TSP50 we generated could be good tools for both basic and clinical studies.
Testis-specific protease 50 (TSP50) has been identified as a testis-specific protein that is expressed abnormally in most human breast cancer samples, which makes it an attractive molecular marker and a potential target for diagnosis and therapy. In the present study, we prepared a panel of monoclonal antibodies (mAbs) with high specificity and sensitivity against TSP50 by hybridoma method and characterized them by ELISA, Western blot, immunofluroescence and immunohistochemicals. The results show that all of the nine different clones can be specifically bind to TSP50. mAbs against TSP50 we generated could be good tools for both basic and clinical studies.