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采用５种诱导培养基和３种分化培养基对４个野生稻种与９个栽培稻品种组配的杂种后代进行花药培养，结果筛选出２种较好的诱导培养基：Ⅰ．籼稻培养基，并以水解乳蛋白３００ｍｇ／ｌ代替水解酪蛋白，甘氨酸２ｍｇ／ｌ代替脯氨酸１００ｍｇ／ｌ；Ⅴ．培养基Ⅰ铁盐加倍，同时表明铁盐加倍对诱导愈伤无显著作用。筛选出１种较好的分化培养基：Ｄ３．ＭＳ培养基＋ＫＴ２＋ＮＡＡ０．５＋水解乳蛋白８００＋肌醇５００（ｍｇ／ｌ）。结果还表明不同基因型的野栽杂种花培愈伤诱导率和绿苗生产力差异很大
Five kinds of induction medium and three kinds of differentiation medium were used for anther culture of hybrid progeny of 4 wild rice varieties and 9 cultivated rice varieties. Two kinds of better induction mediums were screened out: Ⅰ. Indica medium, hydrolyzed casein with hydrolyzed milk protein 300mg / l, glycine 2mg / l instead of proline 100mg / l; Iron I doubled in culture medium Ⅰ, and at the same time it showed that doubling iron salts had no significant effect on inducing callus. Screen a better differentiation medium: D3. MS medium + KT2 + NAA0.5 + hydrolyzed milk protein 800 + myo-inositol 500 (mg / l). The results also show that different genotypes of wild-type hybrid flower callus induction rate and green seedling productivity vary greatly