目的:建立小儿豉翘清热颗粒中黄芩苷含量的测定方法。方法:采用高效液相色谱(HPLC)法,色谱柱为Alltima C_(18)柱(250mm×4.6mm,5μm),流动相为甲醇-水-磷酸溶液(47:53:0.2),流速为1.0ml·min~(-1),检测波长为274nm。结果:黄芩苷在1.20～10.80μg·ml~(-1)浓度范围内与峰面积线性关系良好(r=0.999 7),平均加样回收率为100.31%,RSD=0.90%(n=5)。结论:建立的方法灵敏度高、准确、重复性好,可用于该制剂的质量控制。 Objective: To establish a method for the determination of baicalin in Xiao Er Qu Qing Qing Granules. Method: HPLC method was used. The column was Alltima C 18 column (250mm×4.6mm, 5μm). The mobile phase was methanol-water-phosphoric acid solution (47:53:0.2), the flow rate was 1.0. Ml·min~(-1), detection wavelength is 274 nm. Results: The linear relationship between baicalin and peak area was good in the range of 1.20～10.80μg·ml -1 (r=0.999 7). The average recovery was 100.31% and RSD was 0.90% (n=5). . Conclusion: The established method has high sensitivity, accuracy and repeatability, and can be used for the quality control of the preparation.