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目的 :新发现的DNA病毒 (transfusiontransmittedvirus简称TTV)被认为是一种新的肝炎病毒。为了研究肝炎患者中TTV的感染情况 ,对 10 8例肝炎患者 (ALT >2 0 0IU/L)进行了TTVDNA的检测。方法 :利用巢式PCR方法扩增TTV基因 ,并对两例TTV分离株的部分基因进行克隆和序列分析。结果 :10 8中有 2 4例TTVDNA阳性 ,阳性率为 2 2 2 % ;在非甲～非庚型肝炎中TTV的阳性率为 3 4 6% ( 9/ 2 6) ;甲型肝炎病例中TTVDNA的阳性率为 9 1%( 1/ 11) ;乙型肝炎病例为 2 3 2 % ( 13 / 5 6) ;丙型肝炎病例 7 1% ( 1/ 14 )。两个TTV分离株 (TTVHN 1AF15 15 3 2 ,TTVHN 2AF15 1683 )的核酸序列与GenBank资料中TTV对应序列的核酸同源性高于 90 %。结论 :TTV病毒可能是非甲～非庚型肝炎的重要致病因子 ,并且能够与甲、乙、丙型肝炎病毒发生重叠感染
Purpose: The newly discovered DNA virus (TTV) is considered a new type of hepatitis virus. To investigate the prevalence of TTV infection in hepatitis patients, TTV DNA was detected in 108 hepatitis patients (ALT> 200 IU / L). Methods: The nested PCR method was used to amplify the TTV gene. Some of the two TTV isolates were cloned and sequenced. Results: Totally 24 of 108 patients had positive TTVDNA, the positive rate was 22.2%. The positive rate of TTV in non-hepatitis A and non-hepatitis G was 34.6% (9/26). In the case of hepatitis A The positive rate of TTVDNA was 91% (1/11), that of hepatitis B was 23.2% (13/56), and that of hepatitis C was 7.1% (1/14). The nucleic acid sequences of the two TTV isolates (TTVHN 1AF15 15 3 2, TTVHN 2AF15 1683) and the TTV-corresponding sequences in GenBank data were over 90%. Conclusion: The TTV virus may be an important virulence factor of non-hepatitis A and non-hepatitis G virus, and can overlap with A, B and C viruses