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利用60 Co-γ射线辐射诱变籼稻恢复系中恢8015,获得了一份窄卷叶突变体Nrl3(t)。与野生型中恢8015相比,突变体叶片显著变窄内卷、株高降低、穗长变短、结实率降低、千粒重降低、粒长粒宽减小。细胞学分析表明,突变体维管束减少和泡状细胞不够饱满是导致叶片变窄卷曲的原因。遗传分析表明该突变体受一对隐性核基因控制,以窄卷叶突变体Nrl3(t)与粳稻品种02428杂交获得的F2分离群体作为定位群体,利用SSR标记和新开发的InDel标记,将窄卷叶基因Nrl3(t)定位于第2染色体长臂标记C9和C10E之间约70.3kb区间内。研究结果为该基因的克隆及进一步揭示水稻窄卷叶形成的分子机理奠定了基础。
A narrow leaf-rolled mutant Nrl3 (t) was obtained by using 60 Co-γ radiation to mutagenize the restorer line Zhonghui 8015 in indica rice. Compared with the wild type Zhonghui 8015, the mutant leaves significantly narrowed and the volume was reduced, the plant height was decreased, the spike length was shortened, the seed setting rate was reduced, the grain weight was reduced, and the grain length and grain width were reduced. Cytological analysis showed that the reduction of vascular bundles and insufficient bubbling of the mutant cells were responsible for the narrowing and curling of the leaves. Genetic analysis showed that this mutant was controlled by a pair of recessive nuclear genes. F2 segregating population derived from a narrow leaf clover mutant Nrl3 (t) and japonica rice 02428 was used as a locus. Using SSR markers and newly developed InDel markers, Narrower leaf gene Nrl3 (t) is located in the interval of about 70.3kb between C9 and C10E on the second chromosome. The results laid a foundation for the cloning of this gene and further revealing the molecular mechanism of rice narrow leaf curl formation.