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介绍一种利用表面张力曲线测定生物表面活性剂发酵液浓度的方法,通过测定不同浓度鼠李糖脂标准溶液的表面张力,制作浓度和表面张力的x-y散点图,再根据希斯科夫斯基经验公式利用Origin软件做x-y散点图的拟合曲线,得到该公式的相关参数,然后利用该经验公式,通过测定稀释后待测发酵液的表面张力,可求得原发酵液的鼠李糖脂浓度。和常用的方法相比,该方法具有快速、简单、准确和测定成本低的优点。
A method for measuring the concentration of biosurfactant fermentation broth by surface tension curve is introduced. By measuring the surface tension of different concentrations of rhamnolipid standard solution, xy scatter plot of concentration and surface tension is made, Based on the empirical formula, Origin software is used to make the fitting curve of xy scatter plot, and the related parameters of the formula are obtained. Based on the empirical formula, the surface tension of the diluted fermentation broth can be obtained, Glycolipid concentration. Compared with the commonly used methods, this method has the advantages of fastness, simplicity, accuracy and low cost of measurement.