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目的 :探讨小细胞肺癌的发生、发展机理 ,提高临床早期对小细胞肺癌患者的诊断水平。方法 :应用免疫组化和聚合酶链式反应—单链构象多态性分析的方法技术 ,对 14例小细胞肺癌的石蜡包埋切片进行研究。结果 :p5 3基因的 5、6、7、8外显子突变率为 5 7.1% (8/14 )。在小细胞肺癌组织中存在着较高的p5 3基因突变率。结论 :p5 3基因突变可能为人类肺癌发生的关键基因。
Objective: To investigate the occurrence and development of small cell lung cancer and to improve the early diagnosis of small cell lung cancer. Methods: Paraffin-embedded sections of 14 patients with small cell lung cancer were studied by immunohistochemistry and polymerase chain reaction-single strand conformation polymorphism analysis. Results: The mutation rate of 5, 6, 7 and 8 exons in p5 3 gene was 51.1% (8/14). There is a high rate of p5 3 gene mutation in small cell lung cancer. Conclusion: p5 3 gene mutation may be the key gene in human lung cancer.