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从泰国一个病人分离获得的原虫,先按Trager等的方法。建立连续培养,在第1次酶谱检测时,发现有两型葡萄糖磷酸同功酶(GPI-1和GPI-2)和两型的腺嘌吟核苷脱氨基酶(ADA-1和ADA-2)。培养2周后,ADA-2的活性带就不再出现。5周后GPI-2带亦消失,这些现象表明,原来的分离物包含着几个细胞系。在培养过程中,有些细胞系得到保留的选择。分离方法是用含10%(容积)洗涤的新鲜红细胞的RPMI 1640将培养物稀释至每0.1ml含1.0或0.5个原虫。取0.1ml滴加在培养板的每个井内,加盖后放蜡烛缸内37℃孵育,过一定时间后,
Protozoa isolated from a patient in Thailand were first treated according to the method of Trager et al. A continuous culture was established. Two types of GPI-GPI-2 and two types of adenine nucleoside deaminase (ADA-1 and ADA- 2). After 2 weeks of incubation, the active bands of ADA-2 are no longer present. After 5 weeks, the GPI-2 band also disappeared. These phenomena indicate that the original isolate contained several cell lines. Some of the cell lines are given the option of keeping in culture. The isolation method is to dilute the culture to 1.0 or 0.5 protozoa per 0.1 ml with RPMI 1640 containing 10% (fresh) washed red blood cells. Take 0.1ml drop in each well of the culture plate, put the candle jar after 37 ℃ incubation, after a certain period of time,