论文部分内容阅读
红掌为原产热带的观赏花卉,低温易受冷害。在低温胁迫下,植物体会产生大量活性氧,清除活性氧的过氧化氢酶基因(AnCAT)在植物抗寒过程中起着重要作用。本文以天南星科花烛属红掌‘阿拉巴马’品种叶片提取的总RNA为模板,通过RT-PCR与RACE扩增,获得了一个1 704 bp的过氧化氢酶(catalase,CAT)基因的cDNA序列,其基因编码区共1 476 bp,编码492个氨基酸,命名为AnCAT(GenBank登录号:JX163298)。用低温胁迫处理红掌植株,叶片最大光化学效率Fv/Fm随着低温胁迫处理时间的延长而逐渐下降,当6℃处理达到36 h时Fv/Fm开始急剧下降,叶片也出现萎蔫状态,表明红掌叶片受到极大的伤害;使用实时荧光定量PCR,对不同低温处理时间下CAT基因在红掌植物叶片中的表达量进行测定分析,结果表明:低温胁迫处理12 h后,AnCAT基因明显上调表达,且表达量达到最高点,表明该基因与红掌抗低温胁迫有一定的关系。
Anthurium for the tropical ornamental flowers, low temperature vulnerable to chilling. Under low temperature stress, plants produce large quantities of reactive oxygen species. Anaerobic catalase (AnCAT) plays an important role in cold tolerance in plants. In this paper, a total RNA of 1 704 bp catalase (CAT) gene was obtained by RT-PCR and RACE amplification using the total RNA extracted from leaves of Anthurium andraeanum (Araceae) The coding region of this gene was 1 476 bp in length, encoding 492 amino acids. It was named AnCAT (GenBank accession number: JX163298). When treated with low temperature stress, the maximum photochemical efficiency (Fv / Fm) of the leaves decreased gradually with the prolonging of cold stress treatment. When the temperature reached 6 h at 36 ℃, the Fv / Fm began to drop sharply, and the leaves showed wilting state. Palm leaves were greatly harmed. The expression of CAT gene in leaves of Anthurium was analyzed by real-time PCR. The results showed that the expression of AnCAT gene was up-regulated 12 h after cold stress , And the expression reached the highest point, indicating that the gene and Anthurium anti-low temperature stress have a certain relationship.