本专利提供一种分离、精制苍耳提取物的方法。该方法稳定、可靠、简单、高效。制得的提取物具有抗菌和抗肿瘤活性,而且保留了原药材的稳定性和活性。在中性条件下用醚反复萃取苍耳热水提取物,醚层用无水Na2SO4处理,干燥并浓缩,用甲醇溶解浓缩物。甲醇溶解液依次采用二氯甲烷-醋 This patent provides a method for separating and refining Xanthium extracts. The method is stable, reliable, simple and efficient. The resulting extract has antibacterial and antitumor activity and retains the stability and activity of the original medicinal material. The Xanthium hot water extract was repeatedly extracted with ether under neutral conditions, the ether layer was treated with anhydrous Na2SO4, dried and concentrated, and the concentrate was dissolved with methanol. Methanol solution in turn using dichloromethane-vinegar