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目的:研究大鼠脑出血后脑组织线粒体功能的变化。方法:以50 μL鼠尾血注入大鼠尾状核制作大鼠脑出血模型,注血后分别于12 h及1、3、7 d处死大鼠,断头,取血肿周围脑组织,测定不同时相点血肿周围组织线粒体功能(琥珀酸脱氢酶活性、呼吸控制率)。结果:出血后12 h线粒体呼吸控制率及琥珀酸脱氢酶活性无明显变化,1 d后呼吸控制率较对照组下降,3 d时线粒体呼吸控制率及琥珀酸脱氢酶活性均较对照组下降,出血后7 d线粒体琥珀酸脱氢酶活性、呼吸控制率则较对照组明显下降(P<0.01)。结论:脑出血早期(尤其在12 h之内)血肿周围组织线粒体功能无明显下降,晚期则明显受损。
Objective: To study the changes of mitochondrial function in brain tissue after intracerebral hemorrhage in rats. Methods: Intracerebral hemorrhage rat models were made by injecting rat tail nucleus with 50 μL rat tail blood. Rats were sacrificed at 12 h, 1, 3 and 7 d after the injection of blood and the brain tissue around the hematoma was decapitated At the same time point around the hematoma mitochondrial function (succinate dehydrogenase activity, respiratory control rate). Results: The mitochondrial respiration rate and succinate dehydrogenase activity did not change 12 h after hemorrhage, and the rate of respiration control decreased one day later than that of the control group, and the mitochondrial respiration rate and succinate dehydrogenase activity were lower than those of the control group 7 days after hemorrhage, mitochondrial succinate dehydrogenase activity and respiratory control rate decreased significantly compared with the control group (P <0.01). Conclusion: In the early stage of intracerebral hemorrhage (especially within 12 h), the function of mitochondria in the perihematoma tissue is not obviously decreased, but obviously in the late stage.