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AIM: To investigate the role of p38 mitogen-activated protein kinase (p38MAPK) in murine experimental autoimmune hepatitis (EAH). METHODS: To induce EAH, the syngeneic S-100 antigen emulsified in complete Freud’s adjuvant was injected intraperitoneally into adult male C57Bl/6 mice. Liver injury was assessed by serum ALT and liver histology. The expression and activity of p38 MAPK were measured by Western blot and kinase activity assays. In addition, DNA binding activities of nuclear factor kappa B (NF-κB) were analyzed by electrophoretic mobility shift assay. The effects of SB203580, a specifi c p38 MAPK inhibitor, on liver injuries and expression of proin? ammatory cytokines (interferon-γ, IL-12, IL-1β and TNF-α) were observed. RESULTS: The activity of p38 MAPK and NF-κB was increased and reached its peak 14 or 21 d after the fi rst syngeneic S-100 administration. Inhibition of p38 MAPK activation by SB203580 decreased the activation of NF-κB and the expression of proin? ammatory cytokines. Moreover, hepatic injuries were improved significantly after SB203580 administration. CONCLUSION: p38 MAPK and NF-κB play an important role in an animal model of autoimmune hepatitis (AIH) induced by autoantigens.
METHODS: To induce EAH, the syngeneic S-100 antigen emulsified in complete Freud’s adjuvant was injected intraperitoneally into adult male C57Bl / AIM: To investigate the role of p38 mitogen-activated protein kinase (p38MAPK) in murine experimental autoimmune hepatitis 6 mice. Liver injury was assessed by serum ALT and liver histology. The expression and activity of p38 MAPK were measured by Western blot and kinase activity assays. In addition, DNA binding activities of nuclear factor kappa B (NF-κB) were analyzed by The effects of SB203580, a specifi c p38 MAPK inhibitor, on liver injuries and expression of proin? ammatory cytokines (interferon-γ, IL-12, IL-1β and TNF-α) were observed. RESULTS: The activity of p38 MAPK and NF-κB was increased and reached its peak 14 or 21 d after the fi rst syngeneic S-100 administration. Inhibition of p38 MAPK activation by SB203580 decreased the activation of NF-κB and the expression of proin? ammator CONCLUSION: p38 MAPK and NF-κB play an important role in an animal model of autoimmune hepatitis (AIH) induced by autoantigens.